Lonza Walkersville Human T Cell Nucleofector Kit

Supplier:  Lonza Walkersville VPA1002

The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human T cells (unstimulated or pre-stimulated).The cell type-specific Optimized Protocol is available in our knowledge database. In this Optimized Protocols the best Nucleofection Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human T Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL), 25 Single use pipettes

Catalog No. NC9082860