Invitrogen™ Human TAFA5/FAM19A5 ELISA Kit

Catalog No.	Quantity
EH445RBX10	10 x 96 Tests

Catalog No. EH445RBX10 Supplier Invitrogen™ Supplier No. EH445RBX10

Description

ELISA

Human TAFA5/FAM19A5 quantitates human TAFA5/FAM19A5 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human TAFA5/FAM19A5.

Acts as a chemokine-like protein by regulating cell proliferation and migration through activation of G protein-coupled receptors (GPCRs), such as S1PR2 and FPR2. Stimulates chemotactic migration of macrophages mediated by the MAPK3/ERK1 and AKT1 pathway. Blocks TNFSF11/RANKL-induced osteoclast formation from macrophages by inhibiting up-regulation of osteoclast fusogenic and differentiation genes. Stimulation of macrophage migration and inhibition of osteoclast formation is mediated via GPCR FPR2. Acts as an adipokine by negatively regulating vascular smooth muscle cell (VSMC) proliferation and migration in response to platelet-derived growth factor stimulation via GPCR S1PR2 and G protein GNA12/GNA13-transmitted RHOA signaling . Inhibits injury-induced cell proliferation and neointima formation in the femoral arteries.

Specifications

• Accession Number: Q7Z5A7
• Assay Range: 15-4000 pg/mL
• Assay Sensitivity: 15 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Chemokine-like protein TAFA-5; FAM19A5; family with sequence similarity 19 (chemokine (C-C motif)-like), member A5; family with sequence similarity 19 member A5, C-C motif chemokine like; family with sequence similarity 19, member A5; protein FAM19A5; QLLK5208; RGD1562115; TAFA protein 5; TAFA5; Tafa-5; Tara-5; UNQ5208; UNQ5208/PRO34524Chemokine-like protein TAFA-5; FAM19A5; family with sequence similarity 19 (chemokine (C-C motif)-like), member A5; family with sequence similarity 19 member A5, C-C motif chemokine like; family with sequence similarity 19, member A5; protein FAM19A5; QLLK5208; RGD1562115; TAFA protein 5; TAFA5; Tafa-5; Tara-5; UNQ5208; UNQ5208/PRO34524;
• Gene ID (Entrez): 25817
• Gene Symbol: TAFA5
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: TAFA5
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min