Invitrogen™ Human TCP-1 delta (aa 392-531) Control Fragment Recombinant Protein

Catalog No.	Quantity
RP101874	100 μL

Catalog No. RP101874 Supplier Invitrogen™ Supplier No. RP101874

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (99%), Rat (99%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-83083. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).

Specifications

• Accession Number: P50991
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Neutralization, Control
• Formulation: PBS, 1M urea with no preservative; pH 7.4
• Gene ID (Entrez): 10575
• Name: Human TCP-1 delta (aa 392-531) Control Fragment
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μL
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 2610204B21Rik; A45; C78323; CCT4; cct4 protein; Cctd; CCT-delta; chaperonin containing t-complex polypeptide 1, delta subunit; chaperonin containing TCP1 subunit 4; chaperonin containing Tcp1, subunit 4 (delta); chaperonin subunit 4 (delta); SRB; Stimulator of TAR RNA-binding; T complex protein 1, delta; T-complex protein 1 subunit delta; TCP-1 delta; TCP-1-delta; Unknown (protein for MGC:134500); zgc:65789; zgc:77192
• Common Name: TCP-1 delta
• Gene Symbol: Cct4
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: GSNKLVIEEAERSIHDALCVIRCLVKKRALIAGGGAPEIELALRLTEYSRTLSGMESYCVRAFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISNILEELVVQPLLVSVSALTLATETVRSILK
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining