Invitrogen™ Human Telomerase ELISA Kit

ELISA

Supplier:  Invitrogen™ EEL019

Catalog No. EEL019

Description

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase (TERT) have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.

Specifications

• Accession Number: O14746
• Assay Sensitivity: 0.1 ng/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: TERT
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Test Time: 1 hrs 20 mins

• Assay Range: 0.16 to 10 ng/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum
• Gene ID (Entrez): 7015
• Interassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Quantity: 96 Tests
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Target: TERT
• Total Assay Time: 3 hrs 30 mins