Gibco™ Human TGF-beta 1 Recombinant Protein

Description

Recombinant Protein

Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in an acidic buffer, such as 40mM acetic acid or 4 mM HCl, to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For long term storage, it is recommended to further dilute in buffer containing a carrier protein, such as 0.1% BSA, and store in working aliquots at -20°C to -80°C. Storage: Store the lyophilized protein at 2-8°C or -20°C, preferably desiccated. Store reconstituted human TGF-beta 1 at ≤-20°C (not in a frost-free freezer). Avoid repeated freeze-thaw cycles.

TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.

Specifications

• Accession Number: P01137
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: 100mM acetic acid with no preservative
• Gene ID (Entrez): 7040
• Molecular Weight (g/mol): 25 kDa
• Name: Human TGF-beta 1
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: 4°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: CED; cytokine; DPD1; LAP; Latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; TGF b; TGF b 1; TGF beta; tgf beta 1; tgf beta1; TGF beta-1; TGF beta-1 protein; TGF β; TGF β 1; TGF β1; Tgfb; TGFB1; TGF-B1; Tgfb-1; TGFbeta; TGF-beta 1; TGF-beta 1 protein; TGFbeta1; TGF-beta1; TGF-beta-1; TGFβ1; Transforming growth factor; transforming growth factor b1; transforming growth factor beta; transforming growth factor beta 1; transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor beta-1 proprotein; transforming growth factor beta-1; transforming growth factor β1; transforming growth factor, beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease); transforming growth factor, beta-1; transforming growth factor-beta 1; transforming growth factor-beta I; transforming growth factor-beta-1; transforming growth factor-beta-1 precursor; unnamed protein product
• Common Name: TGF beta-1
• Gene Symbol: Tgfb1
• Biological Activity: ED50 < 0.2 ng/mL; determined by the dose-dependent inhibition of mouse HT-2 cells stimulated with mouse IL-4.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human TGF-beta 1 recombinant protein is a homodimer containing two 112 amino acid subunits
• Content And Storage: 4°C
• Activity: ED50 < 0.2 ng/mL; determined by the dose-dependent inhibition of mouse HT-2 cells stimulated with mouse IL-4.
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Wet Ice
• Expression System: HEK293 cells
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: TGFs (Transforming Growth Factors)
• Research Category: Immunology, Neurobiology, Oncology, Stem Cell Research, Cardiovascular Research, Differentiation, Bone Research
• Product Line: Gibco™
• Purity or Quality Grade: ≥95% by SDS-PAGE