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Gibco™ Human TGF-beta 1 Recombinant Protein

Catalog No. PHG9214
Encompass_Preferred
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Quantity:
10 μg
5 μg

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This lyohpilized protein should be reconstituted in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C to -80°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01137
For Use With (Application) Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
Formulation 100 mM acetic acid with no preservative
Gene ID (Entrez) 7040
Molecular Weight (g/mol) 25 kDa
Name Human TGF-beta 1
Purification Method Sequential Chromatography
Quantity 10 μg
Endotoxin Concentration <0.1 ng/ μg
Gene Alias CED; cytokine; DPD1; LAP; Latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; TGF b; TGF b 1; TGF beta; tgf beta 1; tgf beta1; TGF beta-1; TGF beta-1 protein; TGF β; TGF β 1; TGF β1; Tgfb; TGFB1; TGF-B1; Tgfb-1; TGFbeta; TGF-beta 1; TGF-beta 1 protein; TGFbeta1; TGF-beta1; TGF-beta-1; TGFβ1; Transforming growth factor; transforming growth factor b1; transforming growth factor beta; transforming growth factor beta 1; transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor beta-1 proprotein; transforming growth factor beta-1; transforming growth factor β1; transforming growth factor, beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease); transforming growth factor, beta-1; transforming growth factor-beta 1; transforming growth factor-beta I; transforming growth factor-beta-1; transforming growth factor-beta-1 precursor; unnamed protein product
Common Name TGF beta-1
Biological Activity ED50 < 0.2 ng/mL; determined by the dose-dependent inhibition of mouse HT-2 cells stimulated with mouse IL-4.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Sequence Human TGF-beta 1 recombinant protein is a homodimer containing two 112 amino acid subunits
Content And Storage 4°C
Endotoxin Level <0.1 ng/μg
Shipping Condition Wet Ice
Expression System HEK293 cells
Protein Family Growth Factors & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype TGFs (Transforming Growth Factors)
Research Category Immunology, Neurobiology, Oncology, Stem Cell Research, Cardiovascular Research, Differentiation, Bone Research
Product Line Gibco™
Purity or Quality Grade ≥95% by SDS-PAGE
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WARNING: Cancer - www.P65Warnings.ca.gov
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