Invitrogen™ Human TGF beta-3 ELISA Kit

Catalog No.	Quantity
EH613RB	96 Tests

Catalog No. EH613RB Supplier Invitrogen™ Supplier No. EH613RB

Description

ELISA

Human TGF beta-3 ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of human TGF beta 3 in cell culture supernatants and serum samples.

Transforming growth factor beta 3 (TGF beta 3) is the third member of the transforming growth factor family of cytokines, which also includes TGF beta 1 and beta 2. These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency associated protein (LAP). Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer. As with all members of the family, TGF beta 3 is highly conserved across species, with mouse and human TGF beta 3 demonstrating 100% sequence homology and cross-species activity.The members of this family can be expressed by most cell types and exert pleiotropic effects, which include the suppression of B- and T-cell effector activity, mediation of tissue healing, and suppression of tumor proliferation. The promotion of CD4+CD25+ T-cell expansion is a newly discovered function of the TGF beta cytokines, and indicates an important role in the protection against autoimmunity.

Specifications

• Accession Number: P10600
• Assay Range: 4.3-1500 pg/mL
• Assay Sensitivity: 4.3 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: ARVD; ARVD1; FLJ16571; H-TGF-b-3; LAP; Latency-associated peptide; LDS5; prepro-transforming growth factor beta-3; RNHF; TGF beta 3; Tgfb3; TGF-B3; Tgfb-3; TGF-beta3; TGF-beta-3; Transforming growth factor; transforming growth factor beta 3; transforming growth factor beta precursor; transforming growth factor beta-3; Transforming growth factor beta-3 proprotein; transforming growth factor, beta 3; transforming growth factor-beta3ARVD; ARVD1; FLJ16571; H-TGF-b-3; LAP; Latency-associated peptide; LDS5; prepro-transforming growth factor beta-3; RNHF; TGF beta 3; Tgfb3; TGF-B3; Tgfb-3; TGF-beta3; TGF-beta-3; Transforming growth factor; transforming growth factor beta 3; transforming growth factor beta precursor; transforming growth factor beta-3; Transforming growth factor beta-3 proprotein; transforming growth factor, beta 3; transforming growth factor-beta3;
• Gene ID (Entrez): 7043
• Gene Symbol: TGFB3
• Interassay CV: 12%
• Intraassay CV: 10%
• Kit Contents: Pre-coated microwell plate, Standard, Biotinylated Detection Antibody, Streptavidin-HRP, Sample Diluent, Wash Buffer Concentrate, Assay Buffer concentrate, Stop Solution, Substrate Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Shipping Condition: Wet Ice
• Storage Requirements: -20°C
• Target: TGF beta-3
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min