Invitrogen™ Human Thimet Oligopeptidase/THOP1 ELISA Kit

Description

ELISA

Human Thimet Oligopeptidase/THOP1 quantitates human Thimet Oligopeptidase/THOP1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Thimet Oligopeptidase/THOP1.

A steady-state level of amyloid beta-peptide (Abeta) represents a balance between its biosynthesis from the amyloid precursor protein (APP) through the action of the beta and gamma-secretases and its catabolism by a variety of proteolytic enzymes. Thimet Oligopeptidase (THOP1) has been shown to promote A-beta degradation. It has also been shown to play a role in antigen presentation, metabolism of neuropeptides under 20 amino acids, and cytoplasmic peptide degradation.

Specifications

• Accession Number: AI131655; AI327041; EC 3.4.24; EC 3.4.24.15; Endo-oligopeptidase A; endopeptidase 24.15; EP24.15; MEPD
• Assay Range: 0.35 to 200 ng/mL
• Assay Sensitivity: 0.9 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 7064
• Gene Symbol: Thop1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Thimet Oligopeptidase
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov