Invitrogen™ Human Thromboxane B2 ELISA Kit

Catalog No.	Quantity
EEL061	96 Tests

Catalog No. EEL061 Supplier Invitrogen™ Supplier No. EEL061

Description

ELISA

The Human Thromboxane B2 (TXB2) ELISA quantitates TXB2 in serum, plasma and other biological fluids Principle of the method: The Competitive Thromboxane B2 (TXB2) ELISA research-use-only kit is designed to quantitatively measure TXB2 in Humans. A TXB2 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human TXB2 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human TXB2 in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Thromboxane B2 is an inactive metabolite/product of thromboxane A2. It itself is not involved in platelet activation and aggregation in case of a wound, but its precursor, thromboxane A2 is. Thromboxane A2 synthesis is the target of the drug aspirin, which inhibits the COX-1 enzyme (the source of thromboxane A2 in platelets).

Specifications

• Assay Range: 78.13-5000 pg/mL
• Assay Sensitivity: 46.88 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Thromboxane
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: TXB2TXB2;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Thromboxane B2
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min