Invitrogen™ Human TIMP-1 ELISA (For Lysates) Kit

Catalog No.	Quantity
EH457RBX5	5 x 96 Tests
EH457RB	96 Tests

Catalog No. EH457RBX5 Supplier Invitrogen™ Supplier No. EH457RBX5

Description

ELISA

Human TIMP-1 quantitates human TIMP-1 in cell lysate, tissue homogenate. The assay will exclusively recognize both natural and recombinant human TIMP-1.

TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.

Specifications

• Accession Number: Clgi; Collagenase inhibitor; collagenase inhibitor 16C8 fibroblast; EG-1; embryogenin-1; EPA; EPO; erythroid potentiating activity; erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; LOC100009047; Metalloproteinase inhibitor; metalloproteinase inhibitor 1; metalloproteinase tissue inhibitor; metalloproteinase tissue inhibitor 1; MMP inhibitor; RP1-230G1.3; TIMP; TIMP 1; TIMP metallopeptidase inhibitor 1; Timp1; TIMP-1; TIMP-1 protein; tissue inhibitor of matrix metalloproteinase-1; tissue inhibitor of metallopeptidase 1; tissue inhibitor of metalloproteinase; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor); tissue inhibitor of metalloproteinase-1; tissue inhibitor of metalloproteinases; tissue inhibitor of metalloproteinases 1; TPA-induced protein; TPA-S1
• Assay Range: 30 to 18,000 pg/mL
• Assay Sensitivity: 30 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates, Tissue Homogenates
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 7076
• Gene Symbol: TIMP1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Tissue Homogenate 50 μL
• Storage Requirements: 2°C to 8°C
• Target: TIMP1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov