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Invitrogen™ Human TIMP-1 ELISA (For Lysates) Kit

Catalog No. EH457RBX10
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EH457RBX10 10 x 96 Tests
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ELISA

TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01033
Assay Range 30 to 18,000 pg/mL
Assay Sensitivity 30 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Cell Lysates, Tissue Homogenates
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 7076
Gene Symbol TIMP1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 50 μL, Tissue Homogenate 50 μL
Storage Requirements 2°C to 8°C
Target TIMP1
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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