Invitrogen™ Human TMPRSS2 ELISA Kit

Catalog No.	Quantity
EEL046	96 Tests

Catalog No. EEL046 Supplier Invitrogen™ Supplier No. EEL046

Description

ELISA

The Human Transmembrane Protease, Serine 2 (TMPRSS2) ELISA quantitates TMPRSS2 in serum, plasma, and other biological fluids The Human Transmembrane Protease, Serine 2 (TMPRSS2) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TMPRSS2 is a protein that belongs to the serine protease family. The protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. Its gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage.

Specifications

• Accession Number: O15393
• Assay Range: 0.16-10 ng/mL
• Assay Sensitivity: 0.1 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: TMPRSS
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: D16Ertd61e; EC 3.4.219; epitheliasin; FLJ41954; MGC6821; Plasmic transmembrane protein X; PP9284; PRSS10; Serine protease 10; Tmprss2; TMPS2; Transmembrane protease serine 2; Transmembrane protease serine 2 catalytic chain; Transmembrane protease serine 2 non-catalytic chain; transmembrane protease, serine 2D16Ertd61e; EC 3.4.219; epitheliasin; FLJ41954; MGC6821; Plasmic transmembrane protein X; PP9284; PRSS10; Serine protease 10; Tmprss2; TMPS2; Transmembrane protease serine 2; Transmembrane protease serine 2 catalytic chain; Transmembrane protease serine 2 non-catalytic chain; transmembrane protease, serine 2;
• Gene ID (Entrez): 7113
• Gene Symbol: TMPRSS2
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: TMPRSS2
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min