Invitrogen™ Human TNF-alpha Recombinant Protein

Catalog No.	Quantity
PIRP75738	1 mg

Catalog No. PIRP75738 Supplier Invitrogen™ Supplier No. RP75738

Description

Recombinant Protein

RP-75738 contains purified recombinant human TNF alpha protein with an N-terminal hexahistidine tag. RP-75738 may be used as a positive control in many immunological assays.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Specifications

• Accession Number: P01375
• Concentration: 0.65 mg/mL
• For Use With (Application): Control, Western Blot
• Formulation: 20 mM tris HCl with 50% glycerol and no preservative; pH 8
• Gene ID (Entrez): 7124
• Name: Human TNF-alpha
• pH Range: 8
• Quantity: 1 mg
• Source: E. coli
• Immunogen: N/A
• Regulatory Status: RUO
• Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1 F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
• Common Name: TNF alpha
• Gene Symbol: TNF
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Amino acids Val77-Leu233 containing an N-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions