Gibco™ Human TNF-alpha Recombinant Protein

Catalog No.	Quantity
PHC3015L	10 μg

Catalog No. PHC3015L Supplier Gibco™ Supplier No. PHC3015L

Description

Recombinant Protein

Carrier-Free Storage: Store the liquid human TNF-alpha at ≤-20°C. Upon thawing, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Specifications

• Accession Number: P01375
• For Use With (Application): Bioactivity
• Formulation: 40mM tris with no preservative; pH 8
• Gene ID (Entrez): 7124
• Molecular Weight (g/mol): 17.5 kDa
• Name: Human TNF-alpha
• pH Range: 8
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
• Common Name: TNF alpha
• Gene Symbol: Tnf
• Biological Activity: ED50 = 0.02 - 0.05 ng/mL; determined by the dose-dependent cytotoxcity on L929 cells in the presence of actinomycin D.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Content And Storage: -20°C
• Activity: ED50 = 0.02 - 0.05 ng/mL; determined by the dose-dependent cytotoxcity on L929 cells in the presence of actinomycin D.
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Dry Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Liquid
• Protein Subtype: TNF (Tumor Necrosis Factor)
• Research Category: Immunology, Signal Transduction, Stem Cell Research, Inflammation, Oncology
• Product Line: Gibco
• Purity or Quality Grade: >95% by SDS-PAGE