Human TNFSF14 Microsample ELISA Kit is a Sandwich-based immunoassay designed for the quantitative measurement of TNFSF14 in serum plasma saliva tissue homogenates cell lysates cell culture supernates and other biological fluids It provides a detection range of 15 63-1000 pg/mL with a sensitivity of 5 4 pg/mL The assay can be completed in approximately 3 5h The procedure follows a The test principle applied in this kit is Sandwich enzyme immunoassay The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFSF14 Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFSF14 Next Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated After TMB substrate solution is added only those wells that contain Human TNFSF14 biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color