Gibco™ Human TRANCE (RANKL) Recombinant Protein

Description

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute sRANKL in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -80°C to -20°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 3 months at -80°C to -20°C. Avoid repeated freeze/thaw cycles.

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Specifications

• Accession Number: O14788
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: 5 mM sodium phosphate with 75 mM NaCl and no preservative; pH 7.6
• Gene ID (Entrez): 8600
• Molecular Weight (g/mol): 20 kDa
• Name: Human TRANCE (RANKL)
• Purification Method: Sequential Chromatography
• Quantity: 10 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: CD254; H-RANK-L; hRANKL2; Ly109l; M-Rank-L; ODF; OPG; OPG ligand; OPGL; OPTB2; Osteoclast differentiation factor; osteoprotegerin ligand; Rankl; rank-l; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; sOdf; srankl; TNF-related activation-induced cytokine; Tnfsf11; TNLG6B; Trance; tumor necrosis factor (ligand) superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6 B; tumor necrosis factor ligand superfamily member 11; Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form; tumor necrosis factor superfamily member 11; tumor necrosis factor-related activation-induced cytokine
• Common Name: RANKL (RANK Ligand)
• Biological Activity: Determined by the dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human TRANCE recombinant protein contains 176 amino acids
• Content And Storage: -20°C
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Other Proteins
• Research Category: Immunology, Bone Research, Signal Transduction, Apoptosis
• Product Line: Gibco™
• Purity or Quality Grade: ≥98% by SDS-PAGE and HPLC

Safety and Handling

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