Invitrogen™ Human Transglutaminase 2/TGM2 ELISA Kit

Description

ELISA

Human Transglutaminase 2/TGM2 quantitates human Transglutaminase 2/TGM2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Transglutaminase 2/TGM2.

Transglutaminase (TGase) catalyses the crosslink of proteins by forming e-(g-glutamyl) lysine isopeptide bonds and requires the binding of Ca2+ for its activity. In mammals, eight distinct TGase isoenzymes have been identified. Tissue transglutaminase (tTGase), also known as TGase 2, has four distinct domains: N-terminal b-sandwich, catalytic core and two C-terminal b-barrel domains. tTGase may have a role in cell death, cell proliferation, cell differentiation, and receptor-mediated endocytosis. In the Alzheimer's disease brain, the elevated tTGase activity is manifested by polymerization of a number of proteins, including Ab peptide, b-amyloid precursor protein and the tau protein, with formation of neurofibrillary tangles.

Specifications

• Accession Number: C polypeptide; C polypeptide, protein-glutamine-gamma-glutamyltransferase; G alpha h; G[a]h; G-ALPHA-h; GNAH; LOC100352148; protein-glutamine gamma-glutamyltransferase 2; protein-glutamine-gamma-glutamyltransferase; RP5-1054A22.2; TG C; TG(C); TG2; TGase C; TGase H; TGase2; TGase-2; TGase-H; TgaseII; TGC; Tgm2; Tissue transglutaminase; tissue-type transglutaminase; transglutaminase; transglutaminase 2; transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase); transglutaminase 2, C polypeptide; transglutaminase C; Transglutaminase H; transglutaminase-2; tTG; tTGas
• Assay Range: 0.82 to 200 ng/mL
• Assay Sensitivity: 0.82 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 7052
• Gene Symbol: TGM2
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: TGM2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov