Biotinylated Human TRBC1 Protein (His, Avitag) is a recombinant fragment of the human T cell receptor beta constant 1 (Asp 1-Ala 144, Accession P01850-1) expressed in HEK293 cells and enzymatically biotinylated for use in T-cell receptor research and assay development. Supplied lyophilized in 25 μg vials.
- Recombinant fragment comprising Asp 1-Ala 144 (Accession P01850-1).
- Expressed in HEK293 (human embryonic kidney) cells.
- C-terminal polyhistidine tag with enzymatic biotinylation at a biotin acceptor peptide.
- Calculated molecular weight 19.9 kDa; migrates at ~23 kDa and ~26 kDa
- Purity greater than 90%
- Endotoxin less than 0.2 EU/μg (LAL / rFC).
- Lyophilized formulation from 0.22 μm filtered PBS, pH 7.4, with trehalose.
- Store lyophilized at -20°C or lower; 12-month stability lyophilized at -20°C to -70°C; reconstituted stability up to 3 months at -70°C under sterile conditions.