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Invitrogen™ Human Troponin C ELISA Kit

Catalog No. EH467RBX5
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ELISA

Human Troponin C quantitates human Troponin C in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Troponin C.

Troponin (Tn) is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments, and as a result of calcium-ion uptake by troponin-C, the elements of the muscle thin filament undergo a series of allosteric changes which allow the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number AI874626; cardiac 3; cardiac troponin C; Cardiac troponin I; CMD1FF; CMD1Z; CMD2A; CMH13; CMH7; cTnC; cTnI; MGC116817; RCM1; slow twitch skeletal/cardiac muscle troponin C; Tn1; TnC; TN-C; tncc; Tni; TNN I3; TNNC; TNNC 1; TNNC1; TNNI3; troponin C type 1 (slow); troponin C, cardiac/slow skeletal; troponin C, slow skeletal and cardiac muscles; troponin C1, slow; troponin C1, slow skeletal and cardiac type; Troponin I; Troponin I cardiac; TroponinI
Assay Range 0.11 to 75 ng/mL
Assay Sensitivity 0.11 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 7134
Gene Symbol Tnnc1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Cardiac Troponin C
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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