Invitrogen™ Human Trypsin 1/PRSS1 ELISA Kit

Catalog No.	Quantity
EH468RBX5	5 x 96 Tests
EH468RBX10	10 x 96 Tests
EH468RB	96 Tests

Catalog No. EH468RBX5 Supplier Invitrogen™ Supplier No. EH468RBX5

Description

ELISA

Human Trypsin 1/PRSS1 quantitates human Trypsin 1/PRSS1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Trypsin 1/PRSS1.

Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screene for and diagnose Cystic Fibrosis.

Specifications

• Accession Number: P07477
• Assay Range: 0.29-200 ng/mL
• Assay Sensitivity: 0.29 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Alpha-trypsin chain 1; Alpha-trypsin chain 2; Anionic trypsin I; anionic trypsin-1; Anionic trypsinogen; beta-trypsin; Cationic trypsinogen; digestive zymogen; MGC120175; MGC149362; nonfunctional trypsin 1; pancreatic trypsin 1; pretrypsinogen I; Protease Serine 1; protease serine 2 preproprotein; protease, serine 1; protease, serine 1 (trypsin 1); protease, serine 2; protease, serine, 1; protease, serine, 1 (trypsin 1); protease, serine, 2; protease, serine, 2 (trypsin 2); PRSS1; PRSS2; PTRYI; RATPTRYI; Serine protease 1; Serine protease 2; TCR V beta 4.1; TRP1; Try1; Try-1; TRY2; TRY4; TRY8; Trygn16; TRYP1; TRYP2; TRYP8; trypsin 1; Trypsin I; Trypsin II; trypsin-1; Trypsin-2; trypsinogen; trypsinogen 1; trypsinogen 16; trypsinogen 2; trypsinogen AAlpha-trypsin chain 1; Alpha-trypsin chain 2; Anionic trypsin I; anionic trypsin-1; Anionic trypsinogen; beta-trypsin; Cationic trypsinogen; digestive zymogen; MGC120175; MGC149362; nonfunctional trypsin 1; pancreatic trypsin 1; pretrypsinogen I; Protease Serine 1; protease serine 2 preproprotein; protease, serine 1; protease, serine 1 (trypsin 1); protease, serine 2; protease, serine, 1; protease, serine, 1 (trypsin 1); protease, serine, 2; protease, serine, 2 (trypsin 2); PRSS1; PRSS2; PTRYI; RATPTRYI; Serine protease 1; Serine protease 2; TCR V beta 4.1; TRP1; Try1; Try-1; TRY2; TRY4; TRY8; Trygn16; TRYP1; TRYP2; TRYP8; trypsin 1; Trypsin I; Trypsin II; trypsin-1; Trypsin-2; trypsinogen; trypsinogen 1; trypsinogen 16; trypsinogen 2; trypsinogen A;
• Gene ID (Entrez): 5644
• Gene Symbol: PRSS1
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Trypsin
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov