Invitrogen™ Human TSG-6 ELISA Kit

Catalog No.	Quantity
EH472RBX10	10 x 96 Tests

Catalog No. EH472RBX10 Supplier Invitrogen™ Supplier No. EH472RBX10

Description

ELISA

Human TSG-6 quantitates human TSG-6 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human TSG-6.

The TSG6 gene is transcribed in normal fibroblasts and activated by binding of the cytokines TNF alpha and IL-1 at AP-1 and NF-IL6 sites in its promoter. TSG-6 is a glycoprotein and a member of the hyaluronan-binding protein family, which includes cartilage link protein, proteoglycan core protein and the adhesion receptor CD44. TSG-6 is highly homologous to CD44, particularly in the hyaluronic acid-binding domain. TSG-6 is found in TNF-treated cells; its expression is rapidly activated by TNF alpha, IL-1 and lipopolysaccharide in normal fibroblasts, peripheral blood mononuclear cells, synovial cells and chondrocytes. The presence of TSG-6 in synovial fluid suggests a possible role in rheumatoid arthritis. TSG-6 forms a stable complex with components of the serine protease inhibitor, inter-alpha-inhibitor (I alpha I)). TSG-6 potentiates the inhibitory effect of l alpha l on the protease activity of plasmin. Through their cooperative inhibitory effect on plasmin, TSG-6 and l alpha l can modulate the protease network and thus inhibit inflammation.

Specifications

• Accession Number: P98066
• Assay Range: 0.2-50 ng/mL
• Assay Sensitivity: 0.2 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Hyaluronate-binding protein; TNF alpha induced protein 6; TNF alpha-induced protein 6; Tnfaip6; Tnfip6; TNF-stimulated gene 6 protein; TSG6; TSG-6; tumor necrosis factor alpha induced protein 6; tumor necrosis factor alpha-induced protein 6; tumor necrosis factor alpha-inducible protein 6; tumor necrosis factor induced protein 6; tumor necrosis factor, alpha induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-inducible gene 6 protein; tumor necrosis factor-stimulated gene-6 proteinHyaluronate-binding protein; TNF alpha induced protein 6; TNF alpha-induced protein 6; Tnfaip6; Tnfip6; TNF-stimulated gene 6 protein; TSG6; TSG-6; tumor necrosis factor alpha induced protein 6; tumor necrosis factor alpha-induced protein 6; tumor necrosis factor alpha-inducible protein 6; tumor necrosis factor induced protein 6; tumor necrosis factor, alpha induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-inducible gene 6 protein; tumor necrosis factor-stimulated gene-6 protein;
• Gene ID (Entrez): 7130
• Gene Symbol: TNFAIP6
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: TNFAIP6
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min