Recombinant Protein;1MG;Human VCAM-1, Fc Tag (VC1-H5253) is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Glu 698 (Accession # P19320-1).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 100.5 kDa. The protein migrates as 120-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human VCAM-1, Fc Tag (Cat. No. VC1-H5253) at 5 μg/mL (100 μL/well) can bind Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) with a linear range of 5-156 ng/mL (QC tested).;Immobilized Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human VCAM-1, Fc Tag (Cat. No. VC1-H5253) with a linear range of 0.3-2 ng/mL (Routinely tested).