Gibco™ Human VEGF-121 Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human VEGF 121 is a 28.4 kDa, disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilation via nitric oxide-dependent pathways. Mouse VEGF-A has several variants, one being VEGF-120. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95%.

Specifications

• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Molecular Weight (g/mol): 28.4 kDa
• Name: Human VEGF-121
• Quantity: 100 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: H-VEGF-121; M-VEGF-121
• Common Name: VEGF-121
• Gene Symbol: VEGFA
• Biological Activity: Determined by its ability to stimulate the proliferation of human umbilical vein endothelial cells (HUVEC). The expected ED50 for this effect is 1.0-5.0 ng/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.