Gibco™ Human VEGF-165 Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water or appropriate buffered solution containing 0.1% BSA to regain full activity. Make any further dilutions of the reconstituted protein in buffered solution containing a carrier protein such as PBS with 0.1% BSA. Storage: Store the lyophilized protein at 2-8°C or -20°C for long term storage, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

Specifications

• Accession Number: P15692
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 7422
• Molecular Weight (g/mol): 40 kDa
• Name: Human VEGF-165
• Purification Method: Sequential Chromatography
• Quantity: 10 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: H-VEGF-165; MVCD1; M-VEGF-165; vascular endothelial growth factor 165 b; vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor; VEGF; VEGF164; VEGF165; Vegfa; VEGF-A; VPF
• Common Name: VEGF-165
• Biological Activity: ED50 < 6 ng/mL; determined by the dose-dependent proliferation of HUVECs.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
• Content And Storage: 4°C
• Classification: Carrier-Free
• Bioassay: Proliferation of HUVECs (Human Umbilical Vein Endothelial Cells)
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Wet Ice
• Specific Activity: 1.0 x 10⁶ - 1.7 x 10⁵ units⁄mg
• Expression System: HEK293 cells
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: VEGF (Vascular Endothelial Growth Factor)
• Research Category: Oncology, Angiogenesis, Stem Cell Research, Differentiation, Inflammation
• Product Line: Gibco™
• Purity or Quality Grade: ≥95% by SDS-PAGE and HPLC

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov