Invitrogen™ Human VEGF-B ELISA Kit

Catalog No.	Quantity
EH481RBX5	5 x 96 Tests
EH481RB	96 Tests

Catalog No. EH481RBX5 Supplier Invitrogen™ Supplier No. EH481RBX5

Description

ELISA

Human VEGF-B quantitates human VEGF-B in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human VEGF-B.

Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.

Specifications

• Accession Number: P49765
• Assay Range: 0.4-100 ng/mL
• Assay Sensitivity: 0.4 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: flt-1/VEGFR1 ligand; vascular endothelial growth factor B; vascular endothelial growth factor B167 precursor; vascular endothelial growth factor B186 precursor; vascular endothelial growth factor-B; Vegfb; VEGF-B; VEGFL; VEGF-related factor; Vrfflt-1/VEGFR1 ligand; vascular endothelial growth factor B; vascular endothelial growth factor B167 precursor; vascular endothelial growth factor B186 precursor; vascular endothelial growth factor-B; Vegfb; VEGF-B; VEGFL; VEGF-related factor; Vrf;
• Gene ID (Entrez): 7423
• Gene Symbol: VEGFB
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: VEGFB
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min