This kit detects VTN (vitronectin) in human serum plasma and tissue homogenates with high sensitivity and precision Designed for quantitative analysis it employs a sandwich ELISA methodology requiring 1-5 hours of incubation and a sample volume of 50-100 l per test The assay demonstrates a dynamic detection range of 1 56 ng/mL to 100 ng/mL with a lower limit of detection at 0 39 ng/mL ensuring reliable measurement of low-abundance targets Absorbance is read at 450 nm providing a standardized approach for researchers to analyze VTN levels in diverse biological samples Ideal for both basic research and clinical applications this kit offers robust performance in studying extracellular matrix proteins cell adhesion mechanisms and disease-related biomarkers