Gibco™ Human Wnt-1 Recombinant Protein

Catalog No.	Quantity
PHC1804	10 μg

Catalog No. PHC1804 Supplier Gibco™ Supplier No. PHC1804

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, distilled water, to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 6 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

Specifications

• Accession Number: P04628
• For Use With (Application): Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 7471
• Molecular Weight (g/mol): 34.8 kDa
• Name: Human Wnt-1
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: BMND16; INT1; Int-1; murine mammary tumor virus integration site; OI15; Proto-oncogene Int-1; proto-oncogene Int-1 homolog; proto-oncogene protein Wnt-1; proto-oncogene Wnt-1; sw; swaying; wingless-related MMTV integration site 1; Wingless-type MMTV integration site 1 homolog; Wingless-type MMTV integration site 1, homolog; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1); Wnt family member 1; Wnt1; Wnt-1
• Common Name: WNT1
• Gene Symbol: WNT1
• Biological Activity: ED50 = 1.5 - 2.5 ng/mL; determined by the dose-dependent enhancement of alkaline phosphatase production by murine ATDC5 cells treated with BMP-2 (200 ng/mL).
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human Wnt-1 recombinant protein contains 343 amino acids
• Content And Storage: -20°C
• Activity: ED50 = 1.5 - 2.5 ng/mL; determined by the dose-dependent enhancement of alkaline phosphatase production by murine ATDC5 cells treated with BMP-2 (200 ng/mL).
• Shipping Condition: Wet Ice
• Expression System: E. coli
• Protein Form: Recombinant
• Form: Lyophilized
• Protein Subtype: Wnt Proteins & Receptors
• Research Category: Signal Transduction, Stem Cell Research, Differentiation, Oncology
• Product Line: Gibco
• Purity or Quality Grade: ≥98% by SDS-PAGE and HPLC

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov