Invitrogen™ Human Wnt-4 ELISA Kit

Catalog No.
EH485RBX10	10 x 96 Tests; HRP

Catalog No. EH485RBX10 Supplier Invitrogen™ Supplier No. EH485RBX10

Description

ELISA

Human Wnt-4 quantitates human Wnt-4 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Wnt-4.

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome.

Specifications

• Accession Number: P56705
• Assay Range: 1.22-300 ng/mL
• Assay Sensitivity: 1.22 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: protein Wnt-4; SERKAL; signal molecule; UNQ426/PRO864; wingless-related MMTV integration site 4; wingless-type MMTV integration site family member 4; wingless-type MMTV integration site family, member 4; Wnt family member 4; WNT4; Wnt-4protein Wnt-4; SERKAL; signal molecule; UNQ426/PRO864; wingless-related MMTV integration site 4; wingless-type MMTV integration site family member 4; wingless-type MMTV integration site family, member 4; Wnt family member 4; WNT4; Wnt-4;
• Gene ID (Entrez): 54361
• Gene Symbol: Wnt4
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: WNT4
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min