Invitrogen™ Human ZFYVE21 (aa 119-192) Control Fragment Recombinant Protein

Catalog No.	Quantity
RP98355	100 μL

Catalog No. RP98355 Supplier Invitrogen™ Supplier No. RP98355

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (82%), Rat (82%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-63165. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

ZF21 was initially identified as protein that could bind to the cytoplasmic tail of MT1-MMP (Membrane-type 1 matrix metalloproteinase), a potent invasion-promoting protease. ZF21 is a member of a protein family characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain and regulates focal adhesions (FAs) and cell movement. Knockdown of ZF21 expression resulted in a delay of FA disassembly following induction of synchronous disassembly of FAs by nocodazole treatment, suggesting that ZF21 is involved in FA disassembly. ZF21 contains a noncanonical pleckstrin homology domain that is a possible therapeutic target to treat metastatic cancer.

Specifications

• Accession Number: Q9BQ24
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Neutralization, Control
• Formulation: PBS, 1M urea with no preservative; pH 7.4
• Gene ID (Entrez): 79038
• Name: Human ZFYVE21 (aa 119-192) Control Fragment
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μL
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 1110013H04Rik; AW558780; C85416; FYVE domain containing 21; HCV p7-transactivated protein 1; HCVP7TP1; ZF21; Zfyve21; Zinc finger FYVE domain-containing protein 21; zinc finger FYVE-type containing 21; zinc finger, FYVE domain containing 21
• Common Name: ZFYVE21
• Gene Symbol: ZFYVE21
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: SGATFLVTFGNSEKPETMTCRLSNNQRYLFLDGDSHYEIEIVHISTVQILTEGFPPGGGNARATGMFLQYTVPG
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining