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Cell Biolabs Inc OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit (Fluorometric), 500 assays
Supplier: Cell Biolabs Inc STA344
Cell Biolabs’ OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit is a simple HTS-compatible assay for measuring hydrogen peroxide concentrations or peroxidase activities in biological samples without any need for pretreatment. In the presence of H2O2 and horseradish peroxidase (HRP), non-fluorescent ADHP (10-Acetyl-3, 7-dihydroxyphenoxazine) is oxidized to the highly fluorescent Resorufin. The probe has less background and greater stability. The ADHP-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used Xylenol Orange (FOX) colorimetric assay for H2O2. The probe can be also used as an ultrasensitive assay for peroxidase activity when H2O2 is in excess. The kit has a detection sensitivity limit of 50 nM (H2O2) or 0.1 mU/mL (Peroxidase). Each kit provides sufficient reagents to perform up to 500 assays, including standard curve and unknown samples.
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