Apexbio Technology LLC Hygromycin B 31282-04-9 500mg

Supplier:  Apexbio Technology LLC A2515500

Hygromycin B is an aminoglycoside antibiotic commonly applied in molecular biology research as a selectable marker for transgenic cell lines It inhibits protein synthesis by targeting the ribosomal 30S subunit impairing ribosomal translocation during translation and inducing misreading of mRNA Structurally it binds within the major groove of a helical region of the 16S rRNA altering the activity of ribosomal ATPase and influencing structural dynamics of the decoding site residues notably positions G1494 and A1408 In laboratory investigations Hygromycin B is routinely employed to select genetically modified bacteria fungi and mammalian cells carrying Hygromycin-resistance genes The IC50 values generally vary by organism and cell type typically ranging from 50 g/mL to 500 g/mL

Catalog No. 50-000-62975