Invitrogen™ IC Fixation Buffer

Catalog No.	Quantity
FB001	100 mL

Catalog No. FB001 Supplier Invitrogen™ Supplier No. FB001

Description

Intracellular fixation buffer for use in flow cytomerty, particularly for fixation in intracellular staining procedures.

Intracellular fixation buffer for use in flow cytomerty, particularly for fixation in intracellular staining procedures. Composition is paraformaldehyde in phosphate buffered saline, pH 7.3.

These Made-to-Order catalog items have a 2 week lead time.

Specifications

• Content And Storage: Store at 2°C to 8°C. Do not freeze.
• Product Type: Fixation Buffer
• Quantity: 100 mL
• Solution Type: Fixation Buffer
• For Use With (Application): Cell Surface Antibodies, Intracellular Cytokine Antibodies
• pH: 7.3
• Shipping Condition: Wet Ice

Frequently Asked Questions (FAQs)

What fixative should I use to fix my cells or tissues for antibody labeling?

Aldehyde-based fixatives (e.g., formaldehyde, glutaraldehyde) crosslink various cellular components, which helps retain proteins and cell morphology, but some antigens can be masked by the crosslinking, requiring antigen retrieval methods to unmask the antigen binding sites. Also, aldehyde-based fixation requires permeabilization to allow entry of antibodies. Organic solvents such as methanol and acetone condense proteins and permeabilize the cells, but cell morphology can be compromised. Surface antigens can be labeled without fixation.

For Research Use Only. Not for use in diagnostic procedures.