Invitrogen™ Human IFN alpha ProQuantum Immunoassay Kit

Description

ELISA

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

Specifications

• Accession Number: P01562
• Assay Range: 0.064 to 5000 pg/mL
• Assay Sensitivity: <0.05 pg/mL
• Conjugate: FAM™
• Detection Method: Taqman™ Real-time PCR
• Immunoassay Kit Format: Proximity-based Immunoassay
• Product Type: ProQuantum
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Protein Quantitation
• For Use With (Equipment): qPCR Instruments
• Gene ID (Entrez): 3439, 3440
• Incubation Time: 1 hr.
• Kit Contents: Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
• Label or Dye: FAM™
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
• Storage Requirements: Frozen at -20°C
• Target: IFN alpha
• Test Time: 30 mins
• Total Assay Time: 2 hrs