INTACT GENOMICS INC ig-Fusion™ Cloning Kit 50 Reactions

Supplier:  INTACT GENOMICS INC 4115

Intact Genomics proprietary ig-Fusion™ cloning technology is a simple, rapid, and highly efficient cloning kit that allows direct cloning of any PCR product(s) into any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high-fidelity Pfu DNA polymerase or other high-fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to the site of fusion. The linearized vector can be generated by PCR or restriction enzymes. Multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step using the ig-Fusion™ cloning kit. Cloning is fast (usually 10-30 min) with more than 95% efficiency.Applications Clone any insert at any site within any vector Restriction enzyme and phosphatase-free system Joining multiple large fragments at once Precise insertion at a desired orientation Rapid and high efficiency with > 95% positive clones

Catalog No. 50-000-83001