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INTACT GENOMICS INC ig Recombinase Polymerase Amplification (RPA) Kit v2, 25 Reactions
Supplier: INTACT GENOMICS INC 3533
The improved IG Recombinase Polymerase Amplification Version 2 Kit (IG RPA Kit v2) amplifies DNA at a single and constant temperature (25°C – 42°C) using a recombinase (e.g. UvsX), primers, a single-stranded DNA binding protein (SSB), and a strand displacing DNA polymerase. T4 UvsX is used in combination with its accessory protein, UvsY. The recombinase interacts with the primers to form nucleoprotein filaments. This complex can bind with homologous double-stranded DNA through a strand exchange. After the exchange, a single-stranded binding protein, T4 gp32, stabilizes the displaced strand. Finally, Bsu/Sau DNA polymerase extends the DNA from the primers, creating a new complete copy of the template, and amplification can continue as in Polymerase Chain Reaction (PCR). IG RPA v2 offers More robust amplification and more specific amplification of the primary target than Version 1 for some applications. It may reduce background amplification of off-target sites.
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