INTACT GENOMICS INC ig Recombinase Polymerase Amplification (RPA) Kit v1, 25 Reactions

Supplier:  INTACT GENOMICS INC 3526

The ig RPA Kit version 1 provides the necessary reagents and enzymes to amplify user-provided DNA template using isothermal Recombinase Polymerase Amplification. It requires no thermocycling with equal or better sensitivity than PCR. RPA is a molecular biology technique that amplifies DNA at a constant temperature using a recombinase, primers, a single-stranded DNA binding protein (SSB), and a strand displacing DNA polymerase. T4 UvsX Recombinase is used with UvsY and primers to cause strand exchange of double-stranded DNA. Then, a single-stranded binding protein, T4 gp32, stabilizes the displaced strand. Finally, Bsu or Sau DNA polymerase extends the DNA from the primers and creates a new complete copy of the template. Iterative amplification ensues as with PCR. RPA is a highly selective and sensitive isothermal amplification technique. No DNA pretreatment is required. It is compatible with a variety of endpoint detection methods, (e.g. lateral flow, real-time fluorescence).

Catalog No. 50-000-82916