Invitrogen™ Mouse IGF-2 ELISA Kit

Description

ELISA

The Mouse Insulin-like Growth Factor 2 (Ms IGF-2) ELISA quantitates Ms IGF-2 in mouse cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IGF-2. Principle of the method The Mouse IGF-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.

Specifications

• Accession Number: P09535
• Assay Range: 2.3 to 150 ng/mL
• Assay Sensitivity: 1500 pg/mL
• Calibration Range: 1500 pg/mL to 150 ng/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Growth Factors & Receptors
• Sample Type: Supernatant
• Specificity: The antibody pair provided in this kit recognizes mouse IGF-II (for Cell Culture).
• Sufficient For: 96 assays
• Target Species: Mouse
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 16002
• Gene Symbol: AL033362,Igf-2,Igf-II,M6pr,Mpr,Peg2
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Development
• Sample Volume: Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: AL033362, Igf-2, Igf-II, M6pr, Mpr, Peg2
• Target: IGF2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins