Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™, Invitrogen™

Mouse Isotype Control

Supplier:  Invitrogen 11471481

Catalog No. 50-112-9608

Description

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control. Applications Reported: FITC Mouse IgG1 K Isotype Control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis. Applications Tested: This Mouse IgG1 K Isotype Control is offered in 2 formats: - μg size: This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

Specifications

• Antigen: Mouse IgG1 kappa
• Clone: P3.6.2.8.1
• Conjugate: FITC
• Gene Alias: IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Not Applicable
• Form: Liquid

• Applications: Control, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Quantity: 50 μg
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Isotype: IgG1 κ

For Research Use Only.