Invitrogen™ Mouse IgG1 Uncoated ELISA Kit with Plates

Catalog No.	Quantity
50-246-298	10 x 96 Tests
50-246-295	2 x 96 Tests

Catalog No. 50-246-298 Supplier Invitrogen™ Supplier No. 885041086

Description

ELISA

The Mouse Immunoglobulin G1 (IgG1) Uncoated ELISA Kit contains pre-matched antibody pairs, plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IgG1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

Specifications

• Assay Range: 3.13-200 ng/mL
• Assay Sensitivity: 3.13 ng/mL
• Content And Storage: Capture Antibody Concentrate
  Detection Antibody Concentrate
  Mouse IgG1 isotype control (standard), lyophilized, 400 ng/mL upon reconstitution
  Phosphate Buffered Saline Concentrate (PBS)
  Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
  Tetramethylbenzidine (TMB) Substrate Solution
  96-well plates included with product Cat. Nos. ending in suffixes -22, -76, -86
• Conjugate: HRP
• Immunoassay Kit Format: Uncoated ELISA Kit with Plates
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183;
• Kit Contents: Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86), Purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 50 μL
• Storage Requirements: 2-8°C
• Synonym: Immunoglobulin G1
• Target: Mouse IgG1
• Test Time: 1 hr 45 min
• Total Assay Time: 24 hr 30 min

Frequently Asked Questions (FAQs)

What type of plates are included in the Ready-SET-Go! ELISA sets?

Corning Costar 96-well plates are included with Ready-SET-Go! ELISA catalog numbers ending in suffixes -22, -86 and -76. These solid 96-well plates are optional with the 10 and 20 plate kit sizes.

What is the sensitivity of your Ready-Set-Go! ELISA Sets?

The sensitivity ranges of our Ready-SET-Go! ELISA sets vary depending on the cytokine/protein being measured. Please refer to the product datasheet for set specific information.

What is the expiration date for your Ready-Set-Go! ELISA Sets?

The majority of our Ready-Set-Go! ELISA Sets are guaranteed for one year from the date of shipment. However, we do have several sets that are guaranteed for 6 months upon date of receipt. Please refer to the Certificate of Analysis for shelf-life information.

What are the storage conditions for your Ready-Set-Go! ELISA Sets?

All of the reagents are to be stored at 4 degrees C upon receipt, except for the recombinant standards. The recombinant standards MUST be stored at or below -80 degrees C to ensure stability and performance.

What do Ready-SET-Go! ELISA Sets contain?

Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
10X Coating Buffer
5X ELISA/ELISPOT Diluent
Detection enzyme: pre-titrated Avidin-HRP
Substrate Solution: 1X TMB (Tetramethylbenzidine) Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: Corning Costar 9018