Mouse IgG2a kappa, APC, clone: eBM2a, Isotype Control, eBioscience™ 

Mouse Isotype Control

Catalog Number	Mfr. No.	Quantity
50-151-65	Invitrogen 17472441	25 Tests
50-151-66	Invitrogen 17472442	100 Tests
50-151-67	Invitrogen 17472481	50μg

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This mouse IgG2a isotype control has been reported for use in flow cytometric analysis.Applications Tested: The APC conjugated mouse IgG2a isotype control is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells and can be used at less than or equal to 1 µg/million cells (100 µL blood). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells and can be used at test size: 5 µL (1 µg)/test per million cells (100 µL blood).Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2µm post-manufacturing filtered.

Specifications

• Antigen: Mouse IgG2a kappa
• Target Species: Not Applicable
• Isotype: IgG2a, kappa
• Monoclonal or Polyclonal: Monoclonal
• Classification: Control
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Regulatory Status: RUO

• Clone: eBM2a
• Conjugate: APC
• Applications: Control, Flow Cytometry
• Format: Liquid
• Type: Isotype Control
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.