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IgM (Mu chain) F(ab ft.)2-Goat anti-Mouse, Functional Grade, eBioscience™ Non-distribution product as customer accommodation.

F(ab ft.)2-Goat Polyclonal Secondary Antibody

Manufacturer:  Invitrogen 16509285

Catalog No. 50-112-9042


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Description

Description

This polyclonal F(ab ft.)2 fragment recognizes mouse IgM. Reactivity to human and bovine has been removed. This polyclonal antibody can be used to crosslink and activate B lymphocytes. Applications Reported: This polyclonal F(ab ft.)2 Goat Anti-Mouse IgM has been reported for use in functional assays (such as B cell activation). Concentrations should be determined empirically for optimal performance in the assay of interest. Applications Tested: F(ab ft.)2 Anti-Mouse IgM, u chain specific Functional Grade Purified has been tested by in vitro stimulation of mouse splenocytes. Endotoxin: Less than 0.5 ng/µg antibody as determined by the LAL assay. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.5 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.
Specifications

Specifications

Mouse IgM (Mu chain)
Polyclonal
Functional Grade
Liquid
Ig
500 μg
4° C
Secondary
Mouse
Flow Cytometry, Functional Assay
1 mg/mL
PBS with no preservative; pH 7.2
F(ab′)2-Goat
Affinity chromatography
RUO
Secondary Antibody
polyclonal
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