IgM (Mu chain) F(ab ft.)2-Goat anti-Mouse, Functional Grade, eBioscience™ 

F(ab ft.)2-Goat Polyclonal Secondary Antibody

Manufacturer:  Invitrogen 16509285

Catalog No. 50-112-9042

Description

This polyclonal F(ab ft.)2 fragment recognizes mouse IgM. Reactivity to human and bovine has been removed. This polyclonal antibody can be used to crosslink and activate B lymphocytes. Applications Reported: This polyclonal F(ab ft.)2 Goat Anti-Mouse IgM has been reported for use in functional assays (such as B cell activation). Concentrations should be determined empirically for optimal performance in the assay of interest. Applications Tested: F(ab ft.)2 Anti-Mouse IgM, u chain specific Functional Grade Purified has been tested by in vitro stimulation of mouse splenocytes. Endotoxin: Less than 0.5 ng/µg antibody as determined by the LAL assay. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.5 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

Specifications

• Antigen: Mouse IgM (Mu chain)
• Classification: Polyclonal
• Conjugate: Functional Grade
• Format: Liquid
• Isotype: Ig
• Quantity: 500 μg
• Storage Requirements: 4° C
• Primary or Secondary: Secondary
• Target Species: Mouse

• Applications: Flow Cytometry, Functional Assay
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Host Species: F(ab′)2-Goat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Secondary Antibody
• Monoclonal or Polyclonal: polyclonal

For Research Use Only.