Invitrogen™ Human IgM ELISA Kit

Catalog No.	Quantity
50-112-5324	96 Tests
BMS2098TEN	10 x 96 Tests

Catalog No. 50-112-5324 Supplier Invitrogen™ Supplier No. BMS2098

Description

ELISA

The Human Immunoglobulin M (Hu IgM) ELISA quantitates Hu IgM in human serum, plasma or cell culture supernatant. The assay will exclusively recognize both natural and recombinant Hu IgM. Principle of the method The Human IgM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

Specifications

• Assay Range: 15.6 to 1,000 ng/mL
• Assay Sensitivity: 1 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3507
• Interassay CV: 3.2%
• Intraassay CV: 5.1
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Immunoglobulin M
• Target: Human IgM
• Test Time: 1 hrs 20 mins
• Total Assay Time: 1 hrs 30 mins