Invitrogen™ Human IL-10 ELISA Kit, High Sensitivity

Catalog No.	Quantity
BMS2152HS	96 Tests

Catalog No. BMS2152HS Supplier Invitrogen™ Supplier No. BMS2152HS

Description

ELISA

The Human High Sensitivity Interleukin 10 (HS IL-10) ELISA quantitates Hu HS IL-10 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-10. Principle of the method The Human HS IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Specifications

• Accession Number: P22301
• Assay Range: 0.39-25.0 pg/ml
• Assay Sensitivity: 0.03 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIFCSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF; CSIF; GVHDS; IL-10; IL10; IL10A; TGIF
• Gene ID (Entrez): 3586
• Gene Symbol: IL10
• Interassay CV: 7.5%
• Intraassay CV: 6.8%
• Kit Contents: Pre-coated microwell plate, Biotinylated Detection Antibody, Streptavidin-HRP, Standard, Control, Sample Diluent, Assay Buffer Concentrate, Amplification Reagent I, Amplification Reagent II, Wash Buffer Concentrate, Substrate Solution, Stop Solution , Adhesive Plate Covers, Biotinylated Detection Antibody, Streptavidin-HRP, Standard, Control, Assay Buffer Concentrate, Amplification Reagent I, Amplification Reagent II, Wash Buffer Concentrate, Substrate Solution, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: CSIF, Cytokine synthesis inhibitory factor, IL-10, Interleukin-10, T-cell growth inhibitory factor
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: IL-10
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr