Invitrogen™ Mouse IL-10 Instant ELISA™ Kit

Catalog No.	Quantity
50-112-5462	128 Tests

Catalog No. 50-112-5462 Supplier Invitrogen™ Supplier No. BMS614INST

Description

ELISA

The Mouse Interleukin 10 (Ms IL-10) ELISA quantitates Ms IL-10 in mouse serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Ms IL-10. Principle of the method The Mouse IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Specifications

• Accession Number: P18893
• Assay Range: 39.1-2500 pg/mL
• Assay Sensitivity: 5.28 pg/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with monoclonal antibody to mouse IL-10, Biotin-Conjugate (anti-mouse IL-10 monoclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
  Aluminum pouch(es) with a mouse IL-10 Standard curve (coloured)
  Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
  Sample Diluent (Use when an external predilution of the samples is needed)
  Substrate Solution (tetramethyl-benzidine)
  Stop Solution (1M Phosphoric acid)
  Adhesive Films
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIFCSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF; CSIF; IL-10
• Gene ID (Entrez): 16153
• Gene Symbol: IL10
• Interassay CV: 10.1%
• Intraassay CV: 6.7%
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: IL-10, Cytokine synthesis inhibitory factor, CSIF
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: -20°C
• Synonym: TGIF, MCSF III, CSIF, B TCGF
• Target: IL-10
• Test Time: 15 min
• Total Assay Time: 3 hr 10 min