Invitrogen™ Human IL-10RB ELISA Kit

Catalog No.	Quantity
EHIL10RBX10	10 x 96 Tests

Catalog No. EHIL10RBX10 Supplier Invitrogen™ Supplier No. EHIL10RBX10

Description

ELISA

The Human Interleukin 10 Receptor Beta (Hu IL-10RB) ELISA quantitates Hu IL-10RB in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-10RB. Principle of the method The Human IL-10RB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Natural killer (NK) cells in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. NK cells are important effectors of innate immunity where they release cytokines, which in turn up-regulate other immunological functions. Monoclonal antibodies have been used to identify different surface antigens present on NK cells. These surface antigens have not only been used to identify NK cells, but also their functionally distinct subsets. The Cluster of Differentiation (CD) nomenclature was established to standardize the naming of NK cells.

Specifications

• Accession Number: Q08334
• Assay Range: 5-3000 pg/mL
• Assay Sensitivity: 5 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Cytokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 6620401D04Rik; AI528744; CDW210B; CRF2-4; CRFB4; cytokine receptor class-II CRF2-4; cytokine receptor class-II member 4; Cytokine receptor family 2 member 4; cytokine receptor family II, member 4; D16H21S58; D21S58; D21S58h; D21S66; IL-10 receptor subunit beta; IL-10R subunit 2; IL-10R subunit beta; Il10r2; IL-10R2; IL10RB; IL-10RB; inte; interleukin 10 receptor 2; interleukin 10 receptor subunit beta; interleukin 10 receptor, beta; interleukin-10 receptor subunit 2; Interleukin-10 receptor subunit beta; RGD15603736620401D04Rik; AI528744; CDW210B; CRF2-4; CRFB4; cytokine receptor class-II CRF2-4; cytokine receptor class-II member 4; Cytokine receptor family 2 member 4; cytokine receptor family II, member 4; D16H21S58; D21S58; D21S58h; D21S66; IL-10 receptor subunit beta; IL-10R subunit 2; IL-10R subunit beta; Il10r2; IL-10R2; IL10RB; IL-10RB; inte; interleukin 10 receptor 2; interleukin 10 receptor subunit beta; interleukin 10 receptor, beta; interleukin-10 receptor subunit 2; Interleukin-10 receptor subunit beta; RGD1560373; CDW210B; CRF2-4; CRFB4; D21S58; D21S66; IL-10R2
• Gene ID (Entrez): 3588
• Gene Symbol: IL10RB
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: IL10RB
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min