Invitrogen™ Mouse IL-11 ELISA Kit

Catalog No.	Quantity
EMIL11X10	10 x 96 Tests

Catalog No. EMIL11X10 Supplier Invitrogen™ Supplier No. EMIL11X10

Description

ELISA

The Mouse Interleukin-11(Ms IL-11) ELISA quantitates Ms IL-11 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-11. Principle of the method The Mouse IL-11 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL.

Specifications

• Accession Number: P47873
• Assay Range: 1-450 ng/mL
• Assay Sensitivity: 1000 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: adipogenesis inhibitory factor; AGIF; il 11; IL10RA; Il11; IL-11; IL11 inhibitor; IL-11ra; ILN; Interleukin; interleukin 11; Interleukin11; interleukin-11; MGC2146; Oprelvekinadipogenesis inhibitory factor; AGIF; il 11; IL10RA; Il11; IL-11; IL11 inhibitor; IL-11ra; ILN; Interleukin; interleukin 11; Interleukin11; interleukin-11; MGC2146; Oprelvekin; IL-11
• Gene ID (Entrez): 16156
• Gene Symbol: IL11
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: IL-11
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: IL-11
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min