Invitrogen™ Mouse IL-12 p40/70 (IL-12B) ELISA Kit

Catalog No.	Quantity
EMIL12BX10	10 x 96 Tests

Catalog No. EMIL12BX10 Supplier Invitrogen™ Supplier No. EMIL12BX10

Description

ELISA

The Mouse InIerleukin-12 p40/70 (IL-12B) ) ELISA quantitates Ms IL-12b in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-12b. Principle of the method The Mouse IL-12b solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). Following protein synthesis, an active heterodimer (p70) and a homoodimer of p40 are formed. IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-γ) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-γ and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

Specifications

• Accession Number: P43431, P43432
• Assay Range: 5-2000 pg/mL
• Assay Sensitivity: 5 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Cytokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 subunit p35; IL-12 subunit p40; Il12a; IL-12A; Il12b; IL-12B; IL-12p35; Il12p40; Il-12p40; ILN; Interleukin; interleukin 12a; interleukin 12b; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Ll12a; p35; p40CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 subunit p35; IL-12 subunit p40; Il12a; IL-12A; Il12b; IL-12B; IL-12p35; Il12p40; Il-12p40; ILN; Interleukin; interleukin 12a; interleukin 12b; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Ll12a; p35; p40; IL-12p35; Il-12a; Ll12a; p35;Il-12b; Il-12p40; Il12p40; p40
• Gene ID (Entrez): 16159, 16160
• Gene Symbol: IL12A, Il12b
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35,IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: IL-12 p40/p70
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min