Invitrogen™ Rat IL-12 p40 ELISA Kit

Catalog No.	Quantity
KRC0122	2 x 96 Tests
KRC0121	96 Tests

Catalog No. KRC0122 Supplier Invitrogen™ Supplier No. KRC0122

Description

Includes

Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

ELISA

The Rat Interleukin 12+p40 (Rt IL-12+p40) ELISA quantitates Rt IL-12+p40 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt IL-12+p40. Principle of the method The Rat IL-12+p40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL12B (IL-12 p40) encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kDa cytokine receptor like subunit encoded by this gene, and a 35 kDa subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.

Order Info

Shipping Condition: Wet ice

Specifications

• Accession Number: Q9R103
• Assay Range: 15.6 to 1000 pg/mL
• Assay Sensitivity: <3 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Rat
• For Use With (Application): Colorimetric
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 64546
• Gene Symbol: Il12a, Il12b
• Interassay CV: 5.1%
• Intraassay CV: 4.2
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Precision: <3 pg/mL
• Quantity: 2 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Target: IL-12 p40
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs

For Research Use Only. Not for use in diagnostic procedures.