Recombinant Protein;25UG;Biotinylated Human IL-12 R beta 1, Fc,Avitag (ILB-H82F7) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 540 (Accession # P42701-1).;This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 85.3 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human IL-12B, His Tag (Cat. No. NK2-H52H7) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12 R beta 1, Fc,Avitag (Cat. No. ILB-H82F7) with a linear range of 10-78 ng/mL (QC tested).;Immobilized Human IL-12B, His Tag (Cat. No. NK2-H52H7) at 2 μg/mL (100 μL/well), can bind pre-mixed increasing concentrations of Anti-IL23A & IL12B MAb (P40 domain) and 0.4 μg/mL (100 μL/well) Biotinylated Human IL-12 R beta 1, Fc,Avitag (Cat. No. ILB-H82F7) with a half maximal inhibitory concentration(IC50) of 0.83 μg/mL (Routinely tested).