Invitrogen™ Mouse IL-17B ELISA Kit

Catalog No.	Quantity
EMIL17B	96 Tests
EMIL17BX5	5 x 96 Tests

Catalog No. EMIL17B Supplier Invitrogen™ Supplier No. EMIL17B

Description

ELISA

The Mouse Interleukin-17B (Ms IL-17B) ELISA quantitates Ms IL-17B in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-17B. Principle of the method The Mouse IL-17B solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies.

Specifications

• Accession Number: Q9QXT6
• Assay Range: 12-12000 pg/mL
• Assay Sensitivity: 12 pg/mL
• Calibration Range: 12 pg/mL to 12000 pg/mL
• Content And Storage: Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Cytokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-13, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-5, M-CSF, MIG, MIP- 1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TPO, VEGF.
• Sufficient For: 96 assays
• Target Species: Mouse
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1110006O16Rik; 1700006N07Rik; Cytokine CX1; Cytokine Zcyto7; Cytokine-like protein ZCYTO7; gene for cytokine-like protein; IL17B; IL-17B; IL20; IL-20; Interleukin; interleukin 17B; interleukin 20; interleukin-17 beta; interleukin-17B; Interleukin20; Interleukin-20; neuronal interleukin-17 related factor; neuronal interleukin-17-related factor; NIRF; UNQ516/PRO1031; ZCYTO71110006O16Rik; 1700006N07Rik; Cytokine CX1; Cytokine Zcyto7; Cytokine-like protein ZCYTO7; gene for cytokine-like protein; IL17B; IL-17B; IL20; IL-20; Interleukin; interleukin 17B; interleukin 20; interleukin-17 beta; interleukin-17B; Interleukin20; Interleukin-20; neuronal interleukin-17 related factor; neuronal interleukin-17-related factor; NIRF; UNQ516/PRO1031; ZCYTO7; 1110006O16Rik; 1700006N07Rik; Zcyto7
• Gene ID (Entrez): 56069
• Gene Symbol: IL17B
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: IL-17B, Cytokine CX1, Cytokine-like protein ZCYTO7, Neuronal interleukin-17-related factor
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Inflammation
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: 1110006O16Rik, 1700006N07Rik, Zcyto7
• Target: IL17B
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min